Systematic Review and Meta-analysis on the Inactivation Rate of Viruses and Bacteriophage by Solar Wavelength Radiation

Sunlight is a known biocide, and photodriven inactivation is an important avenue for controlling viruses in both natural and engineered systems. However, there remain significant unknowns regarding damage to viruses by sunlight, including the impact of wavelength and viral characteristics. Herein, a systematic review of the literature and meta-analysis was conducted to identify inactivation rate constants (k-values) when exposed to solar wavelengths (280–700 nm) for common human viruses and surrogates in natural and synthetic matrices. We identified 457 k-values, with 356 for nonenveloped viruses. Extracted rate constants were transformed into UV fluence-normalized k-values to isolate the most photobiologically relevant wavelengths in the solar spectrum and reported for the first time in terms of energy, rather than time, based units. Each spectral region was assessed independently, with UVB illumination reporting the highest inactivation rates, UVA contributing to inactivation both in the presence and absence of photosensitizers, and visible light demonstrating no biocidal activity. Inactivation mechanisms are reviewed identifying knowledge gaps in translating UVC mechanisms to longer wavelengths. The data compiled in this meta-analysis can be applied to inform the environmental transport of viruses, estimate solar disinfection performance in variable light conditions, or design disinfection systems based on UVA and UVB light.