Scott_Metabolizable_protein_in_transition_dairy_cows

Figure S1. Horizontal barplot showing differentially abundant metabolic pathways identified by LEfSe analysis between 80MP and 100MP (baseline) fed animals. Pathways are ranked by their Linear Discriminate Analysis (LDA) score (log base 10 scale), with higher scores indicating a greater effect size. Colours representing different microbial families significantly associated with each pathway; Figure S2. Correlation matrix for key pathways of short chain fatty acid metabolism and methane production in the rumen with abundant rumen families identified with metagenome-assembled genomes. Positive correlations are shown in red, negative correlations in purple, with asterisks denoting statistical significance (* P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001). Supplemental Table 1. Rumen ASV table for abundance; Supplemental Table 2: Milk ASV table for abundance