Supplementary Material for: Expression of the Inhibitory CD200 Receptor Is Associated with Alternative Macrophage Activation

Classical macrophage activation is inhibited by the CD200 receptor (CD200R). Here, we show that CD200R expression was specifically induced on human in vitro polarized macrophages of the alternatively activated M2a subtype, generated by incubation with IL-4 or IL-13. In mice, peritoneal M2 macrophages, elicited during infection with the parasites Taenia crassiceps or Trypanosoma brucei brucei, expressed increased CD200R levels compared to those derived from uninfected mice. However, in vitrostimulation of mouse peritoneal macrophages and T. crassiceps infection in IL-4–/– and IL-4R–/– mice showed that, in contrast to humans, induction of CD200R in mice was not IL-4 or IL-13 dependent. Our data identify CD200R as a suitable marker for alternatively activated macrophages in humans and corroborate observations of distinct species- and/or site-specific mechanisms regulating macrophage polarization in mouse and man.

经典巨噬细胞活化受到CD200受体（CD200R）的抑制。本研究揭示，在体外极化的人巨噬细胞中，M2a亚型的CD200R表达在经IL-4或IL-13孵育后特异性诱导。在感染绦虫类原虫粗梳绦虫或锥虫属布鲁斯氏锥虫的鼠类中，腹腔M2巨噬细胞相较于未感染鼠类表现出CD200R水平的升高。然而，在IL-4–/–和IL-4R–/–小鼠的腹腔巨噬细胞体外刺激及粗梳绦虫感染实验中，与人类相比，小鼠CD200R的诱导并非依赖于IL-4或IL-13。本研究数据将CD200R确认为人类M2型巨噬细胞表型选择的适宜标记物，并证实了小鼠与人类在巨噬细胞极化调节机制上存在物种特异性及/或部位特异性差异的观察结果。