In-house database specific to bacteria from horses for MALDI Biotyper

The dataset contains the main spectral profiles (MSPs) for MALDI Biotyper CA 3.2 System (Bruker Japan, Kanagawa, Japan) constitutes in-house database specific to bacteria from horses for identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS is used for identification of bacterial species isolated from horses. however, some bacterial species isolated from horses are difficult to identify with MALDI-TOF MS because of insufficiencies in the reference database, and enriching the databases is expected to enhance the accuracy of MALDI-TOF MS identification. Here we created an in-house database including 271 bacterial isolates from horses. Bacterial isolates were subjected to ethanol / formic acid treatment, and spectra were gained using flexControl 3.4 software (Bruker Japan). The spectra were checked with flexAnalysis 3.4 software (Bruker Japan) to delete spectra differing from the cohort spectra and imported for generating MSPs using MBT Compass Explorer 4.1.7.0. software (Bruker Japan). MSPs were exported in a *.btmsp format for use with MALDI Biotyper systems.