How Does Hydrophobicity Change of Nonionic Surfactant Influence the Co-adsorption of Monoclonal Antibody and Surfactant at the Amphiphilic Self-assembled Interface?

We have previously found that nonionic surfactants, such as Polysorbate 80 (commonly denoted as PS80 or Tween 80), could form a monolayer on amphiphilic UV-TMOS treated surface, greatly inhibiting mAb from further adsorption. We speculate that the hydrophilic heads of PS80, which are adsorbed on the outside of the UV-TMOS interface, prevent further adsorption of the mAbs. This aroused our interest to investigate whether the hydrophobicity of surfactants, especially headgroup size, would impact the co-adsorption of mAb and surfactant.