Gene expression analysis for Oregon R embryos at different developmental stages.. Drosophila melanogaster

The transcription profiles of newly mapped Drosophila heterochromatic sequences during early embryonic stages were investigated by microarray. Overall design: cDNA were prepared from manually staged wild type Oregon R embryos at various developmental stages (0-1 hr, 2-2.5 hr/cycle 13-early cycle 14, 2.5-3 hr/mid- to late cycle 14, 3-4 hr, 4-5 hr, 19-22 hr) and analyzed by microarray. cDNA prepared from Oregon R embryos with mixed developmental stages (0-16 hrs) were used as reference for hybridization. Each stage has two biological repeats. 100-150 embryos were collected for each experiment. All embryos were collected at room temperature.