PD-L1 regulates dendritic cell ferroptosis during immunogenic chemotherapy [scRNA-seq]

Dendritic cells (DCs) express high levels of PD-L1 in the tumor microenvironment and draining lymph nodes. Here, we explore the roles of PD-L1 signaling during immunogenic chemotherapy. DC-conditional PD-L1 knockout mice were inoculated with MC38-OVA tumors and treated with doxorubicin. T cells were isolated from draining lymph node for single cell RNA sequencing. DC-conditional PD-L1 knockout mice were inoculated with MC38-OVA tumor cells. After tumor established, mice were treated with doxorubicin. Draining lymph nodes were isolated and single cell suspensions were prepared. Live T cells were sorted by flow cytometry and single cell libraries were prepared.