Early-life exposure to tobacco smoke alters airway signaling pathways in D. melanogaster and later mortality in D. melanogaster

To investigate the effect of cigarette smoke exposure on gene expression in airway epithelial cells of Canton S Drosophila melanogaster larvae, we isolated the airways of cigarette smoke exposed larvae and air controls. We then performed gene expression profiling analysis using data obtained from RNA-seq of smoke-exposed males, smoke-exposed females, air-control males and air-control females. For each group 4 biological replicates were prepared, representing 40-50 larval airways. Comparative gene expression analysis of RNA-seq data for isolated trachea of smoke exposed and air controls of Canton S Drosophila melanogaster larvae, separated into males and females