Bigger Is Better Than Many: A Strategy to Optimize Multi-Gene Co-Expression - Data

This study challenges the long-held assumption that larger plasmids are less efficient for gene delivery. Using both experimental data and modeling, the authors show that consolidating multiple genes onto a single larger plasmid achieves higher co-transfection efficiency and more coordinated gene expression than multi-plasmid systems, making it a more reliable strategy for multi-gene expression in mammalian cells.