Raw data on uterine fluid proteomics in buffaloes

These data are related to a study that aimed to compare the uterine fluid (UF) of pregnant and non-pregnant buffaloes and describe the relationship between embryo and uterus during early pregnancy. Forty-four females were submitted to a hormonal estrus synchronization and randomly divided into two groups: pregnant (n = 30) and non-pregnant (n = 14). The pregnant group was artificially inseminated and divided into two groups: P12 (n = 15) and P18 (n = 15), in which embryo and uterine fluid samples were collected 12 and 18 days after the insemination during slaughter. The non-pregnant group was also redivided into two groups at the synchronization end: NP12 (n = 7) and NP18 (n = 7). The UF samples were processed for proteomic analysis. Samples were prepared for proteomics (ESI Q-TOF MS/MS).

本数据集与一项旨在比较妊娠与非妊娠水牛的子宫液（UF），并描述早期妊娠期间胚胎与子宫之间关系的研究相关。共选取了44头雌性水牛进行激素诱导发情同步处理，并随机分为两组：妊娠组（n = 30）与非妊娠组（n = 14）。妊娠组经过人工授精后，进一步分为P12组（n = 15）和P18组（n = 15），在屠宰时分别于授精后的第12天和第18天收集胚胎及子宫液样本。非妊娠组在同步处理结束时也分为NP12组（n = 7）和NP18组（n = 7）。子宫液样本经过蛋白质组学分析处理。蛋白质组学分析中，样本制备采用电喷雾离子化-四极杆飞行时间串联质谱（ESI Q-TOF MS/MS）技术。