Genome-wide identification of AP-2α targets in bladder cells by RNA-seq (Control vs AP-2α KD)

TFAP2A (AP-2α) is a member of the transcription factor AP-2 family, functions as a tumor suppressor by regulating the expression of various cancer-related genes; its expression is positively correlated with chemosensitivity and survival of cancer patients. SV-HUC-1 cells were infected with a lentivirus expressing scramble shRNA or AP-2α shRNA for 48 h and then the cells were selected with puromycin (1 µg/mL). Cells infected with scramble shRNA (control) and AP-2α shRNA were sequenced by Illumina HiSeq2000.