Datasets and R script

A model ectotherm organism (larvae of the European Common Frog) was exposed to two ubiquitous stressors - elevated temperatures resulting from global warming and nitrate pollution - in a crossed experimental design. Health biomarkers were associated to underlying changes in larvae gut bacteria. Bacteria composition and predicted metabolic pathways corroborated faster developed at higher temperatures and reduced body condition under nitrate pollution. Microbiome adjustments seem to have buffered damages to larvae health to a certain extent. However, the highest levels of both stressors culminated in reduced body condition and hampered ability to accelerate development to escape a stressful environment.