Identification of differentially expressed genes by rapid induction of PML/RARa degradation using arsenic trioxide in NB4 cells

Acute promyelocytic leukemia (APL) is characterized by the specific chromosome translocation t(15;17) that generates the promyelocytic leukemia/retinoic acid receptor-a (PML/RARa) fusion gene. Arsenic trioxide has been intensively reported to directly and rapidly degrade the PML/RARa fusion protein. Here, we treated NB4 cells (a widely used APL cell line) with arsenic trioxide and performed RNA-seq analysis to identify differentially expressed genes upon arsenic trioxide treatment. Overall design: RNA-seq analysis of NB4 cells with or without arsenic trioxide treatment.