Qualitative results for the detection of virus in tissues collected from challenged or contact exposed pigs by virus re-isolation, immunohistochemistry (IHC) and RRT- PCR.

−/−/−  =  Virus re-isolation/IHC/PCR.
*Lymph nodes, IHC: Superficial inguinal, lateral retropharyngeal**, medial retropharyngeal, right, middle and left tracheobronchial**, mediastinal and mesenteric (Virus re-isolation and PCR only).
Other: Commodities and offal - spleen, liver, kidney and ileum plus muscle (longissimus dorsi and biceps femoris) were all negative when tested by virus re-isolation & PCR.
***Only in cell debris in lumen of three bronchioles (no bronchial or bronchiolar epithelial or macrophage immunolabelling).
Shaded rows represent a mock-infected control animal from the equivalent of dpi 3 and a transmission cycle (TC4) animal at 11 days post-contact (dpc) representing for comparative purposes the closest animal to the direct infected group PMEs.