Overloading mesenchymal progenitors (FAPs)

Skeletal muscle possesses the ability to adapt its size in response to milieus, which is called plasticity. Resistance training induces the increment of muscle mass called muscle hypertrophy. Muscle stem cells (MuSC; also known as muscle stem cells) function to supply new nuclei for myofiber during the overload in muscle. We found that Yap1 and Taz in mesenchymal progenitors (also called FAPs) are critical for MuSC proliferation in overloaded muscles. We hypothsize that Yap1/Taz-dependent mesenchymal progenitors derived factor induces MuSC proliferation. In order to identify such factors, RNA-seq of overloaded FAPs were performed. Overall design: Sham and overloaded mesenchymal progenitors from Control (Pdgfra-CreERT2) or cdKO (Pdgfra-CreERT2::Yap1 flox/flox ::Taz flox/flox) were used for RNA-seq analysis.