Stromal inflammation is a targetable driver of hematopoietic aging [Microarray analysis]

We performed integrated analyses of hematopoietic stem and progenitor cells as well as bone marrow stromal cells to better understand the underlying mechanisms of blood aging. To determine how the molecular state of multipotent progenitor (MPP) cells change with age we performed transcriptomic analyses of myeloid-biased MPP3 and lymphoid-biased MPP4 populations from young and old mice. Microarray analysis of 3-5 biological replicated of the indicated MPP populations were isolated from young (8-10 weeks) and old (24 months) wild-type C57BL/6 mice. MPP3s are identified as Lin-/cKit+/Sca-1+/Flk2-/CD48+/CD150-, and MPP4s are identified as Lin-/cKit+/Sca-1+/Flk2+. Young controls listed here are also available under GSE68529.