Genome-wide analyses of early translational responses to elevated temperature in Oryza sativa (CBX138). unidentified

We investigated genome-wide changes in mRNA translation in Oryza sativa. cv.nipponbare suspension cell cultures exposed to brief periods of stress: elevated temperature(41??). To this end, we subjected polysomal RNA and non-polysomal RNA from scrose gradient fractionated cell lysates to the co-hybridization on Agilent rice Oligo Microarrays. The ratio of signal intensities (polysomal RNA: non-polysomal RNA) was used as am indicator of the translation state for each trascript. To inspect coordination of changes in translational profiles with transcriptional profiles, we also isolated total RNAs from the same cells used for traslational profiling experiments and investigated changes in accumulated transcript levels in respose to stress using the microarray. Two biological replicates were analysed