Impaired Liver X Receptor alpha phosphorylation affects hepatic chromatin H3K27 acetylation patterns

Liver X Receptors (LXRa and ß) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess how changes in the phosphorylation status of LXRa affect the H3K27Ac histone modification, a known marker for active regulatory elements in promoters and enhancers, in livers of mice mice that carry a whole-body phosphorylation deficient-version of LXRa (S196A) compared to wild-type (WT), in response to a high fat-high cholesterol diet. Overall design: High-throughput profiling of the H3K27Ac histone modification in liver of wild-type (WT) and LXRa-S196A female mice after being fed a High Fat-High Cholesterol diet for 6 weeks.