Single-cell RNA sequencing of thioglycollate-elicited peritoneal cells from myeloid-specific S1pr1 gain- and loss-of-function mice

Sphingosine-1-phosphate receptor 1 (S1pr1) plays an important role in myeloid cell trafficking and inflammatory responses. Here, single-cell RNA sequencing was performed on thioglycollate-elicited peritoneal cells isolated from mice with myeloid-specific S1pr1 gain-of-function or knockout, as well as their corresponding Cre-negative controls. This dataset enables interrogation of S1pr1-dependent transcriptional programs across inflammatory myeloid populations, with downstream analyses focusing on neutrophils. Overall design: Thioglycollate was administered to elicit inflammatory peritoneal cells in mice. Single-cell suspensions were prepared from the peritoneal cavity and subjected to single-cell RNA sequencing. Mice included myeloid-specific S1pr1 gain-of-function and knockout models generated using LysM-Cre, along with Cre-negative littermate controls. Although multiple myeloid populations were captured, downstream analyses focused on neutrophil subsets identified based on canonical marker expression.