The novel lysine deacetylase ABHD14B regulates transcription of metabolic genes

Purpose: To understand the role of ABHD14B in cellular metabolism by regulation of transcription. To address this, we performed a RNA-seq experiment with stable knockdowns of ABHD14B in HEK293T cells generated by lentivirus-based shRNA technology. Methods: Four biological replicates of HEK293T wild-type control, non-targeting shRNA control (NT), and two knockdowns (KD_2 and KD_3) of ABHD14B cells were collected and total RNA was extracted in QIAzol. The total RNA was used to generate a cDNA library using Quantseq 3' mRNA kit Fwd. Results: Transcriptional profiling revealed that genes involved in cellular metabolism are differentially regulated in HEK293T ABHD14B knockdown cells. Conclusions: Our study demonstrates the first extensive transcriptomics analysis of mammalian cells upon knocking down ABHD14B, and finds that loss of ABHD14B results in altered expression of glucose and lipid metabolic genes. This observation was complemented by changes in the levels of key intermediates of glucose metabolic pathways and triglycerides. Together, our findings illuminate an important metabolic role played by the KDAC ABHD14B. Four biological replicates of HEK293T wild-type control, non-targeting shRNA control (NT), and two knockdowns (KD_2 and KD_3) of ABHD14B cells were collected and total RNA was extracted in QIAzol. The total RNA was used to generate a cDNA library using Quantseq 3' mRNA kit Fwd.