Microglial replacement reverses age-associated epigenetic modifications despite accelerating epigenetic age (extended datasets)

Microglial replacement is emerging as a promising approach for treating age-related disorders, but effects on epigenetic age remain unclear. We examined epigenetic changes in microglia influenced by age, stroke, and microglial replacement. Old microglia showed an aged epigenetic profile. Stroke and microglial depletion/repopulation, both driving proliferation, led to accelerated epigenetic age. However, at the genome-wide level, repopulation reversed many aging-related changes, particularly those linked to immune and inflammatory response. This repository contains supplementary information and extended datasets mentioned in the original article.