Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion dependent mechanism

BACKGROUNDB-type lamins are critical nuclear envelope proteins that interact with the three-dimensional genomic architecture. However, identifying the direct roles of B-lamins on dynamic genome organization has been challenging as their joint depletion severely impacts cell viability. To overcome this, we engineered mammalian cells to rapidly and completely degrade endogenous B-type lamins using Auxin-inducible degron technology.RESULTSUsing live-cell Dual Partial Wave Spectroscopic (Dual-PWS) microscopy, Stochastic Optical Reconstruction Microscopy (STORM), in situ Hi-C, CRISPR-Sirius, and Fluorescence in situ hybridization (FISH), we demonstrate that lamin B1 and lamin B2 are critical structural components of the nuclear periphery that create a repressive compartment for peripheral-associated genes. Lamin B1 and lamin B2 depletion minimally alters higher-order chromatin folding but disrupts cell morphology, significantly increases chromatin mobility, redistributes both constitutive an..., , , # Lamin B1, B2, and B1/B2-AID Hi-C Files

## Description of the data and file structure

Hi-C files from Pujadas et al., 2024. (*Under Review*) All related files/ codes are available at

In this dataset, there is an Excel file entitled, \"LMNB_project_Hi-C_wasabi_links\" that contains all of the links to FASTQs and processed files for each experiment on Wasabi. **Column A** shows the name of the Hi-C data set that is related to the specific auxin treatment condition (e.g., untreated, 24-hour auxin, and auxin withdraw) for LMNB1, LMNB2, and LMNB1/B2 degradation using the AID system. There are also two replicates of a 48-hour auxin treatment condition for LMNB1/B2 degradation. **Column B** shows the name of the condition for the uploaded .HiC files. While not all of the .HiC files have been uploaded to Dryad, all of the conditions and .Hi-C files (both raw and processed) can be obtained using using the links in this spreadsheet. As the Hi-C data was obtained using a paired-end run, one R...