Gene expression count matrix for 4 T cell subtypes from ROSMAP participants

Peripheral blood mononuclear cells (PBMCs) from participants in the Rush Religious Orders Study/Memory and Aging Project (ROSMAP) were isolated by Ficoll gradient centrifugation, then sorted by high-speed flow cytometry into the following T cell subtypes: CD4+CD45RO-, CD4+CD45RO+, CD8+CD45RO-, and CD8+CD45RO+. Total RNA was extracted using buffer TCL (Qiagen), then RNA-seq libraries were prepared according to the Single Cell RNA Barcoding and Sequencing method originally developed for single-cell RNA-seq, adapted for extracted total RNA. RNA libraries were collected on a single 384-well plate and sequenced on the Illumina HiSeq using the High-throughput 3’ Digital Gene Expression (DGE) library. The "RNA count matrix" file is the raw counts from the 384-well plate, while the "ROSMAP_Tcell_DGE_PlateMap" file contains metadata for the wells on the plate, by well position.