Cartridge for the Retention of All Pathogens

These contents contain the design and methods for using the 3D-printed Cartridge for Retention of All Pathogens (CRAP). CRAP was designed and used by members of the University of Guelph wastewater surveillance team. CRAP is a low-cost, rapid, and easy method to concentrate pathogens from water. CRAP is designed to contain a Keurig K-cup that holds a paper coffee filter. Although it uses an atypical filter, we hypothesized that it effectively captures solid-partitioning pathogens in wastewater.