Maternal Stress During Pregnancy Imprints Fetal Lung Group 2 Innate Lymphoid Cells

We investigated the gene expression on the feral lungs (E18) in the offspring of dexamethasone (DEX)- or PBS- treated mothers by single cell RNA sequence. Overall design: Fetal lungs were harvested on gestation day 18 from each of two mice that had received water containing DEX or PBS since gestation day 12. Fetal lungs (DEX-1, n = 8; DEX-2, n = 9; PBS-1, n = 5; PBS-2, n = 8) were pooled per sample. Fetal lungs cells were prepared by single-cell suspension protocol of fetal lungs.