Numerical data for systemic effects of photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) porphyrin on healthy Drosophila melanogaster

Porphyrins are commonly used as photosensitizing agents (PA) in photodynamic therapy (PDT), a non-invasive method predominantly used for the treatment of subcutaneous tumors. Development of novel PA with increased tissue selectivity and effectiveness is important for the wider use of PDT in the treatment of various types of cancers.  We used Drosophila melanogaster as a model organism to study the systemic effects of 5,10,15,20-tetrakis(N-methylpyridinium-3-yl)-porphyrin (TMPyP3). First, we defined the optimal feeding protocol and showed that TMPyP3 was absorbed and retained longer in the neuronal than the non-neuronal extracts. Hydrogen peroxide (H2O2) concentration increased 24 hours after the photoactivation of orally delivered TMPyP3 but only in the head extracts. After 7 days, regardless of the photoactivation, TMPyP3 led to lower concentration of H2O2, which correlated with the decreased climbing ability measured as negative geotaxis. The results suggest that systemic treatment wi..., Stability of TMPyP3 in nutrient media - figure 3.A
We sampled 25 mg of M1, M2 and M3, and dissolved them in distilled water in duplicates to a final volume of 1 mL. Samples were heated for 30 minutes at 77 °C and centrifuged for 10 minutes at 15 000 rpm. 200 µL of each media supernatant in triplicate (with and without TMPyP3) was added to a 96-well plate, and fluorescence emission spectra of TMPyP3 was recorded using excitation wavelength of 420 nm and emission in 550-800 nm range.
TMPyP3 concentration in the tissue after the oral administration - figure 3.B, 4A,B and 5A,B
To determine the amount of TMPyP3 that was present in the whole body, we prepared a whole-body homogenate immediately after feeding. Using lysis buffer consisting of 1 x phosphate-buffered saline (1 x PBS) at pH 7.4 in the presence of 0.1% (v/v) Triton X-100 (Sigma Aldrich). The volume of extraction buffer was determined as the proportion of 300 µL of buffer and 5 mg tissue sample. NaCl, KCl, Na2HPO4, and KH2PO4, were..., , # Numerical data for systemic effects of photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) porphyrin on healthy Drosophila melanogaster

#### The first sheet in Excel called PDT is the results generated for figure three in the publication (F3).

**Data generated under part F3.A** refer to measurements of **fluorescence** **emission spectra of TMPyP3** was recorded using excitation wavelength of 420 nm and emission in 550-800 nm range. Medium 1 (**M1**) was a standard nutrient medium (73 mL tap water, 4.5 mL molasses, 1.1 g table sugar, 2.6 g inactivated yeast, 0.9 g type II agar, 0.75 mL propionic acid (Sigma Aldrich, 99%) and 0.75 mL of 10 % p-hydroxybenzoic acid methyl ester (NIPAGINE, Roth, 99%) dissolved in 95% ethanol (VWR). Medium 2 (**M2**) was a combination of agar, sugar, and yeasts (75 mL tap water, 1.5 g type II agar, 3 g table sugar and 6.9 g inactivated yeast).Medium 3 (**M3**) was agar and sugar without yeasts (75 mL tap water, 1.5 g type II agar, 3 g table sugar...