Short-read and long-read RNA sequencing of mouse haematopoietic stem cells at bulk and single-cell levels

Hematopoiesis starts with a population of self-renewing hematopoietic stem cells (HSCs), which produce a series of increasingly more lineage-committed progenitor cells, ultimately giving rise to all types of mature blood cells. Here we characterised transcriptomic profiles of LT- and ST- HSCs, as well as multipotent progenitor cells (MPP) using both short- and long-read RNA sequencing methods.