IP STING - Identification of new partners

To access STING function in absence of inflammation, given the tight interconnexion between metabolic and immune pathways, we questioned the impact of Sting ablation on metabolic parameters in vivo. Mice deficient for STING presented profound modifications of glucose management and body temperature. To identify the molecular mechanism through which Sting regulates metabolic homeostasis, we performed tandem-affinity purification of Flag- and HA-tagged Sting (F/HA-Sting) stably expressed in mouse embryonic fibroblasts (MEF) knockout for Sting (MEF Sting-/- ). Immunopurified material was analyzed by Mass Spectrometry to identify Sting protein partners.

鉴于代谢途径与免疫途径之间紧密的互联性，在无炎症状态下访问STING功能时，我们质疑了STING基因敲除对小鼠胚胎成纤维细胞（MEF Sting-/-）中稳定表达Flag和HA标记的STING（F/HA-Sting）的代谢参数的影响。STING基因敲除的小鼠表现出显著的葡萄糖代谢和体温调节的改变。为了揭示STING调控代谢稳态的分子机制，我们对小鼠胚胎成纤维细胞中表达的STING（MEF Sting-/-）进行了串联亲和纯化，并通过质谱分析免疫纯化物质，以鉴定STING蛋白的相互作用伙伴。