NetworKIN predicted active kinases.

Phosphorylation sites from the two proteomic studies were analysed using the NetworKIN algorithm and the predictions were filtered based on kinases detected in the AT1aR-HEK kinome. NetworKIN verified the activity of many of the kinases found by phospho-antibody array by Xiao et al., 2010 and predicted activity for several other kinases, of which around half have been described in the literature of angiotensin II signalling. Numbering of amino acids is according to SwissProt. Amino acids marked * are involved in the regulation of the kinase (SwissProt). Kinases that have not previously been described to be regulated in the angiotensin II signaling pathway are depicted in bold. Phosphorylation sites detected in either of the two proteomic studies Christensen et al., 2010 and Xiao et al., 2010 are denoted Ref. 4 and 5, respectively.