Early epigenetic changes precede genetic alterations and drive hepatocarcinogenesis. Early epigenetic changes precede genetic alterations and drive hepatocarcinogenesis

genome-wide methylome and transcriptome profiling of cirrhotic liver, low- and high-grade dysplastic lesions, eHCC and pHCC synchronouCLy detected in HCC patients with chronic hepatitis B infection. Identification of molecular epi-drivers by integrative analyses and validation in two independent cohorts comprising 933 HCC. Overall design: A total of 28 samples were collected including 7 surrounding liver tissues, i.e. cirrhotic liver (CL), 4 low-grade dysplastic nodules (LGDN), 9 high-grade dysplastic nodules (HGDN), 5 early HCC (eHCC) and 3 progressed HCC (pHCC) from 8 HCC patients with chronic hepatitis B infection.