The effect of THADA knockdown on Huh7 cells. Homo sapiens
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下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA273458
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We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells. Overall design: The pGIPZ shRNA plasmid specific to human THADA (clone ID V3LHS_318037 Open Biosystems, Inc.) or the pGIPZ non-silencing shRNAmir lentiviral plasmid (as a control) was cotransfected with the pPACKH1 plasmid mix in HEK 293 cells. Stable clones of Huh7 cells with THADA knockdown were routinely maintained in a medium containing of puromycin (500ng/ml)
创建时间:
2015-01-23



