The effect of THADA knockdown on Huh7 cells. Homo sapiens

We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells. Overall design: The pGIPZ shRNA plasmid specific to human THADA (clone ID V3LHS_318037 Open Biosystems, Inc.) or the pGIPZ non-silencing shRNAmir lentiviral plasmid (as a control) was cotransfected with the pPACKH1 plasmid mix in HEK 293 cells. Stable clones of Huh7 cells with THADA knockdown were routinely maintained in a medium containing of puromycin (500ng/ml)