The role of histone H3K27 acetylation for enhancer activity in embryonic stem cells. The role of histone H3K27 acetylation for enhancer activity in embryonic stem cells

By substituting lysine 27 in histone variant H3.3 to arginine in mouse embryonic stem cells, we measured H3K27ac/H3K4me1/H3K4me3 and other acetylation modifications in H3.3K27R mutant cells, together with RNA-seq and ATAC-seq to measure the transcription activity and chromatin accessibility, respectively. Overall design: WT mouse ESC served as controls , and two H3.3K27R mutant lines (Mut18 and Mut43) were sequenced. Two independent biological replicates were measured for ChIP-seq / RNA-seq /ATAC-seq experiments, and input data were shared among histone ChIP experiments for each cell line.