Tumor-infiltrating CCR2+ inflammatory monocytes counteract specific immunotherapy

We used Single-cell RNA-sequencing to analyze the composition of the tumor microenvironment of MC38mOVA tumors upon transcutaneous immunization with our immunization method DIVA. MC38mOVA tumor cells were inoculated s.c. into WT mice. Mice were treated by transcutaneous immunization at day 6/7 and 13/14 after inoculation or left untreated. At day 16, respectively day 20, single cell suspensions were prepared from excised whole tumors, and CD45+ cells were enriched by magnetic bead separation. Afterwards Single-cell RNA-sequencing of these CD45+ cells was performed.