Figure_2_replicates

(B) Xrn1 knock out A549 cells were infected with WT or PR8-PA(∆X), or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel.  (D-F) HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters carrying a duplicate cut site from the reporter itself, or additional sequences from the STOML2 and YKT6 genes (99 bp around putative cut sites), and where indicated, with PA-X from the PR8 (D-F), H1N1pdm09 or Perth influenza strains (F). RNA was extracted to run 5’ RACE (D, F) or northern blotting (E).For all DNA gels, the DNA bands were purified and sequenced to confirm their identities. R## --> replicate number; LG###/MMG#### --> experiment number. sgd files --> original source files obtained with Syngene imager