Isl1 identifies the extraembryonic mesodermal / allantois progenitors and required for placenta morphogenesis and vasculature formation [CUT&Tag]

To uncover direct targets of ISL1 that could account for its role in allantois, we performed cut tag analysis on wild type allantoic cells using ISL1 antibody at E8.75. Our analyses uncovered 5509 significant binding peaks for ISL1, mainly located at intergenic or intronic regions. Cut-tag analysis was performed using the allantois of pregnant mice with Black Swiss background with Rosa-tdTomato background in order to trace Isl1 Cre lineage at E8.75. Tissue samples were dissected under fluorescence microscope.