Expression profiling of miR-142 deficient Treg cells

miR-142 gene is specifically and abundantly expressed in hematopoietic lineages. Mice with specific deletion of this miRNA gene in Treg develop fatal autoimmunity. We used RNA-seq to detect whole transcriptome changes in miR-142 null Treg cells. Overall design: Total RNA from sorted CD4+YFP+ Treg cells (Foxp3Cre-YFP,n=3 and Foxp3Cre-YFPmiR-142fl/fl, n=3) was extracted and subjected to RNA-seq. Samples in Foxp3Cre and Foxp3CremiR-142fl/fl groups are biological replicates and were isolated in age and gender matched mice.