Assessment of desiRm on experimentally verified mismatched siRNAs of siPrnp102(T9).

siPrnp102(T9) and its various mutant siRNAs were targeted against prion protein genes (PRNP) and its mutant allele (PRNP-P102L). Mutated base in siRNA is denoted by small letter while mismatch base between siRNA and target are denoted by bold letter. Data of actual efficacy of siRNAs were taken from experimental work reported by Ohnishi et al[36]. Predicted efficacy denotes efficacy of desiRm. All sequences are in 5′ to 3′ direction. Correlation coefficient between actual and predicted efficacy is R = 0.725.