Hepatocyte growth factor(HGF) and insulin-like growth factor-1(IGF-1) for repair of bone-cartilage defects in bone marrow mesenchymal stem cells in a rabbit model of postmenopausal

In postmenopausal osteoporosis (PMOP), the differentiation of bone marrow mesenchymal stem cells (BMSCs) is unbalanced,because of the lack estrogen, that is, osteogenic differentiation is reduced, adipogenic differentiation is increased.To investigate the mechanism of aberrant differentiation of BMSCs in PMOP, we performed transcriptome sequencing of Bone marrow mesenchymal stem cells in a postmenopausal osteoporosis model(PMOP-BMSCs)and Bone marrow mesenchymal stem cells in a healthy control model(Normal-BMSCs).In this analysis, the reference transcriptome sequencing of 6 samples was completed, and the genome alignment of each sample was obtained by aligning the reads to the reference genome, and the expression of protein-coding genes was analyzed based on the alignment results. According to the expression of protein-coding genes in different samples, differential screening was performed, and a total of 1 differential group was set up. We established an induced postmenopausal osteoporosis model using the method of removing bilateral ovaries;Then, the bone marrow mesenchymal stem cells of the osteoporosis rabbit model and the bone marrow mesenchymal stem cells of normal control rabbits were analyzed by RNA-seq for gene expression profiling；Comparative gene expression profiling of RNA-seq data from bone marrow mesenchymal stem cells for osteogenic, chondrogenic and adipogenic differentiation