Elucidation of the mescaline biosynthetic pathway in peyote

Peyote is an entheogenic and medicinal cactus native to the Chihuahuan desert. The psychoactive and hallucinogenic properties of peyote are principally attributed to the phenethylamine derivative mescaline. Despite the isolation of mescaline from peyote over 120 years ago, the biosynthetic pathway in the plant has remained undiscovered. Here, we use a transcriptomics and homology-guided gene discovery strategy to elucidate a near-complete biosynthetic pathway from L-tyrosine to mescaline. We identified a cytochrome P450 that catalyzes the 3-hydroxylation of L-tyrosine to L-DOPA, a tyrosine/DOPA decarboxylase yielding dopamine, and four substrate-specific and regiospecific substituted phenylethylamine O-methyltransferases. Biochemical assays with recombinant enzymes or functional analyses performed by feeding putative precursors to engineered yeast (Saccharomyces cerevisiae) strains expressing candidate peyote biosynthetic genes were used to determine substrate specificity, which served ..., Pacbio Hifi reads of peyote was processed and assembled by Hifiasm into contig level assembly. To enable differential metabolomics and transcriptomics analysis, peyote buttons were dissected into (i) epidermal and chlorenchyma tissue, referred to as the ‘outside’ (OUT) fraction, and (ii) a vasculature and cortex fraction, referred to as the ‘inside’ (IN) fraction. Total IN and OUT RNA libraries were sequenced on an Illumina NovaSeq 6000 system with pair-end reads of 150 bp. Transcriptomes were assembled using Trinity., , This archive contains materials related to the paper \"Elucidation of the mescaline biosynthetic pathway in peyote (Lophophora williamsii)\", by Watkins et al. There are 2 types of data here:

* genome: genomic sequencing data, contig level assembly of the peyote genome.
* transcriptome: transcriptomes of the IN and OUT tissues of peyote buttons.

Please contact [[e-mail]](mailto:qiushi.li@ucalgary.c) with any questions.