Flow cytometry dataset of dexamethasone and immune checkpoint blockade in 4T1 breast cancer model (Ki67 and FoxP3)

Flow cytometry analyses were conducted on cells isolated from tumors and draining lymph nodes (dLNs) of 4T1-bearing mice to assess lymphocyte proliferation. Ki67 staining was used as a proliferation marker together with surface and intracellular markers to define T cell subsets. Cells were stained with Zombie Aqua for viability, PE–Foxp3, PE-CF594–conjugated F4/80, Ly6G, and B220 (dump channel), PerCP-Cy5.5–CD4, APC/Cy7–CD8, Pacific Blue–CD45, and PE/Cy7–Ki67. Tumor-infiltrating lymphocytes and dLN-resident T cells were gated and analyzed for Ki67 expression under dexamethasone, immune checkpoint blockade (ICB), or combination treatment conditions. These data provide insights into how dexamethasone influences the proliferative capacity of T cells in both the tumor microenvironment and secondary lymphoid tissues.