Antibodies used in this study.

Information about all used antibodies in this study is reported including the epitope recognized, the antibody supplier, the catalog number, the lot number and the dilution used in Western Blot procedure. Antibodies were divided in 3 categories: primary monoclonal antibodies, primary polyclonal antibodies and secondary antibodies. Membranes were incubated overnight (ON) or over 2 days (2d) with primary antibodies at 4°C. The following day, membranes were washed 3 times and then incubated with a horseradish peroxidase-coupled secondary anti-mouse/rabbit antibody that recognizes both heavy chain (HC) and light chain (LC) of Igs, or TrueBlot ULTRA antibody, or light chain (LC)-specific antibody. Information regarding the amount of homogenate proteins loaded per lane for each antibody, as well as the incubation time for primary and secondary antibodies is also reported. We classified monoclonal antibodies according to their non-specificity against endogenous Igs; high non-specificity (black), moderate (grey), and absent (white). Antibodies in white can be detected with conventional secondary anti-mouse antibodies (HC+LC) because they do not display non-specificity. Antibodies in grey display low non-specificity and secondary anti-mouse antibodies can be used (HC+LC). Eventually, antibodies in black display high non-specificity and TrueBlot or anti-LC secondary antibodies are necessary to detect tau signal.