Heterogeneity of hematopoietic progenitors in adult mouse liver

To investigate whether liver-resident ILC1s could develop from local hematopoietic progenitors, we analyzed the phenotypic properties of liver CD45+Lin- progenitors. We found that the adult mouse liver contained Lin-Sca-1+Mac-1+ (LSM) hematopoietic progenitors derived from the fetal liver. This population included Lin-CD122+CD49a+ progenitors that could generate liver ILC1s but not conventional NK (cNK) cells. By performing single-cell RNA seq, we show the heterogeneous composition of these hematopoietic progenitors. Purified liver LSM cells and Lin-CD122+CD49a+ cells from adult WT mice were stained with 0.4% Trypan blue to check their viability, and processed for scRNA-seq (10X Genomics).