Trinotate annotations of the transcriptome assemblies of Anchomenus dorsalis, Amara similata, Harpalus affinis, Harpalus eous, Pterostichus melanarius, Poecilus cupreus, Aphidius rhopalosiphi, Zabrus tenebrioides

Functional annotation of the transcriptome assemblies generated by Trinity for Anchomenus dorsalis, Amara similata, Harpalus affinis, Harpalus eous, Pterostichus melanarius, Poecilus cupreus, Aphidius rhopalosiphi, and Zabrus tenebrioides was performed with Trinotate (v4.0.2) (Bryant et al. 2017). Long open reading frames and peptide sequences derived were obtained using the Perl scripts TransDecoder.LongOrfs and TransDecoder.Predict, respectively (Haas, BJ. https://github.com/TransDecoder (v5.7.0)). Trinotate pipeline relies on several softwares: Diamond (v2.0.15) (Buchfink et al. 2021) to identify homology to the UniProt database (The UniProt Consortium 2025), HMMER (v3.3.2) to identify protein domain (Finn et al. 2011), TMHMM (v2.0c) to predict transmembrane domains (Krogh et al. 2001), SignalP (v4.1) to predict secretory signal peptide sites (Petersen et al. 2011), Infernal (v1.1.2) to identify non-coding RNAs using Rfam database (Kalvari et al. 2018), and eggNOG mapper (v2.1.9) to predict gene ontology terms for each transcript (Cantalapiedra et al. 2021). Output of each software were gathered in an SQLite database and Trinotate reports were generated for each species and are available in the compressed file trinotate_annotations.tar.gz.