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ES-derived Dlx6a-Cre fate-mapped neuron bulk RNA-seq

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NIAID Data Ecosystem2026-03-14 收录
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Parental ES reporter line Dlx6a-Cre; Ai9 was differentiated using 2 different transcriptional specifiation strategies: 1) Nkx2-1/Dlx2 or 2) Nkx2-1/Dlx2/St18. Neurons were directeed towards neural, telencephalic and then ventral telencephalic identity prior to neuronal differentiation and reporter gene activation. Cells were differentiated for 12 days. tdTomato+ neurons were isolated by flow cytometry prior to RNA isolationa and library construction. Comparison of DIFF12 N/D and N/D/S tdTomato+ neurons by bulk RNA-seq

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2023-01-06
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