In situ method for the determination of nutrient acquisition and its hormonal regulation in the spiral valve of two chondrichthyan fishes

This dataset describes the validation of a novel dual perfusion of the spiral valve intestine of two condrichthyan species, the Pacific spiny dogfish (Squalus suckleyi) and the Pacific spotted ratfish (Hydrolagus colliei). Perfusate was circulated through the vasculature of the spiral valve via the anterior and posterior intestinal arteries and 3H-glucose or 3H-oleic acid was accumulated from the spiral valve lumen into the anterior and posterior intestinal veins. Further validation of the preparation demonstrated sodium dependence of glucose transport as well as an effect of bovine insulin administration to the arterial circulation.