Transcriptome assembly for nanopore sequencing

We evaluated the performance of multiple leading transcriptome assembly algorithms on their ability to accurately reconstruct RNA transcript isoforms. We specifically focused on deep nanopore sequencing of synthetic RNA spike-in controls (Sequins™ and SIRVs) across different chemistries, including cDNA and direct RNA protocols. Genomic and transcriptomic files deposited here constitute the input data to our analysis called "Assembly Arena: Benchmarking RNA isoform reconstruction algorithms for nanopore sequencing".