RNA-seq analysis of transcriptomes of Noc4L deficient and the Nco4L sufficient Treg cells

To gain in depth transcriptional information about the Noc4L deficient and Noc4L sufficient Tregs, we performed RNA-seq analysis to compare the transcriptomes of Noc4L deficient (CD4+GFP+CD62Lhigh, cKO) and Noc4L sufficient (CD4+GFP+CD62Lhigh, Het) Tregs activated in vitro with Dynabeads for 48 hours or 72 hours. We found ~25 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 48 hours in vitro. And ~100 unique genes with differential expression between the Noc4L deficient and Noc4L sufficient Treg cells when activated for 72 hours in vitro in two times experiment. Transcriptional profiles of Noc4L sufficient (CD4+GFP+CD62Lhigh,Het) and Noc4L deficient (CD4+GFP+CD62Lhigh,cKO) Treg cells isolated from Foxp3CreNoc4l+/fl (Het) or Foxp3CreNoc4lfl/fl (cKO) mice then activated with Dynabeads for 48 hours or 72 hours were generated by RNA-sequencing.