Global gene transcription analysis in response to changes in chromosome supercoiling in Streptomyces coelicolor

Here, we address the transcriptional response to both novobiocin-induced rapid chromosome relaxation or long-term topological imbalance, both increased and decreased supercoiling, in environmental antibiotic-producing bacteria belonging to the Streptomyces genus. RNA was extracted from vegetative hyphae of Streptomyces coelicolor growing for 18h in liquid YEME/TSB medium (30 mL). To induce supercoiling changes S. coelicolor culture was treated with novobiocin in final concentration 10 g/ml for 10 min (rapid chromosome relaxation). For long-term exposure to topological imbalance we used PS04 stain in which TopA level is controlled by thiostrepton addition (long-term high and low DNA supercoling in not induced and induced PS04 strain, respectively)