Transformation of Gonipterus sp. n. 2 embryos with the CRISPR/Cas9 gene editing tool

Literature review discusses the insect sex determination pathway and CRISPR/Cas9 gene editing tool in application for genetic-based pest control methods. The de novo genome of Gonipterus sp. n. 2 was sequenced with long and short read sequencing, assembled and annotated using RNA sequencing data and Protein sequences from other Coleopteran species with published, assembled genomes. The expansion and contraction of gene families, with a focus on the cytochrome P450 detoxification gene, was assessed. The ground work was laid for a CRISPR/Cas9 transformation protocol for Gonipterus sp. n. 2 embryos with microinjection. The Tyrosine 3-monooxygenase (Pale) gene was targeted to assess the efficiency of the embryo transformations.

文献综述探讨了昆虫性别决定途径及CRISPR/Cas9基因编辑工具在基于遗传的害虫控制方法中的应用。通过对Gonipterus sp. n. 2的全新基因组进行长读长和短读长测序，并结合RNA测序数据以及其他已发表、组装完成的鞘翅目物种的蛋白质序列进行组装和注释。对基因家族的扩增与收缩进行了评估，重点关注了细胞色素P450解毒基因。为Gonipterus sp. n. 2胚胎的CRISPR/Cas9转化方案奠定了基础，并通过显微注射技术进行转化。针对酪氨酸3-单加氧酶（Pale）基因进行了靶向研究，以评估胚胎转化的效率。